NCI Frederick, MD
Supports the planning and execution of research experiments in the context of the laboratory's overall research program.
· Independently direct and conduct special research projects that have a high priority for the laboratory with special emphasis in the development of novel research treatments
· Work with animal models e.g., zebrafish and mouse and/or tissue cultures e.g., cell lines and induced pluripotent stem cells, iPSC; use molecular biology and genetics techniques e.g., RT-PCR, in situ hybridization, Western blotting, and immunohistochemistry, morpholino and CRISPR-Cas9-sgRNA gene knockdown
· Apply standard animal techniques such as genotyping and husbandry
· Manage colony of mice for genetics experiments, including setting up mating pairs, genotyping mice and harvesting tissue embryos
· Develop, design and recommend further experimental work and approaches to the research
· Perform forward and reverse genetic and pharmacological screenings
· Design and conduct experiments and studies requiring the application of a broad professional knowledge of scientific theories and principles
Evaluates, analyzes and interprets research data, communicates results and methods in reports, presentations, and manuscripts.
· Write technical reports and prepare manuscripts.
· Identify outcomes relevant to the research
· Acquire, organize, and analyze experimental data and perform statistical analysis
· Maintain a detailed and up-to-date electronic laboratory notebook detailing all experiments
Coordinates with junior staff to modify research protocols.
· Coordinate projects being developed in the laboratory, proposing research strategies and monitoring their progress.
· Meet regularly to train and oversee experiments of junior trainees (e.g., summer interns and IRTA students)
· Work with staff to confirm assay results and evaluate new techniques and instruments; search literature; propose, design and test alternative assays for difficult screening targets; confirm assay results; develop secondary assays
· Suggest alternative or complementary approaches to experiments
Presents research at national scientific meetings and symposia.
· Write papers and abstracts detailing research efforts and results for publication in peer-reviewed journals; prepare PowerPoint presentations as appropriate.
· Review, edit, and, as needed, write first drafts of papers, posters and manuscripts.
· Prepare oral presentations and posters for internal and external groups conferences
· Co-author manuscripts on research projects; publish in peer-reviewed journals
- Molecular biology, including molecular cloning, DNA/RNA purification, polymerase chain reaction (PCR, including qPCR, RT-PCR, RT-qPCR)
- Animal handling, genotyping: Zebrafish
- Animal handling, genotyping: Mouse
- Cell culture techniques
- Gene expression analysis, including in situ hybridization, immunofluorescence/immunohistochemistry, Western blotting
- Data analysis, presentation
- Tissue dissection and sectioning
- Manuscript writing
- CRISPR design/injection (zebrafish)
- Microscopy (light/fluorescent)
- Ordering supplies
- MS Office applications (Outlook, PowerPoint, Word, Excel)
- Reference management software (e.g., Endnote)
- Software for figure creation such as Adobe Illustrator or Adobe Photoshop
- Adobe Acrobat
· MS degree in developmental biology, genetics or molecular biology is required; Ph.D. degree is desirable
· Prior experience animal models including zebrafish and mouse
The world famous “Building 10” ON NIH’s main campus in Bethesda Maryland