Location: Bethesda, MD
Type: Direct Hire
Job #34982

ROLES / RESPONSIBILITES

Prepare sheath fluid and reagents needed for the instruments in the Core facility
Perform daily calibration, operation and maintenance of Becton Dickinson (BD) flow cytometry cell analyzers including LSRII, LSRFortessa, LSRFortessa X20 and SymphonyA5.
Perform calibration, operation and maintenance of BD FACSAria, BD Symphony A6, and BD FACS FUSION cell sorters.
Perform electronic cell sorting of cell populations based on surface markers and/or intracellular probes.
Collaborate with staff on performing complex multiparameter analysis, real time calcium flux analysis and DNA content analysis.
Perform multicolor data acquisition using BD FACSDiva.
Provide assistance to facility users in instrument operation and troubleshooting.
Manage Flouorchrome-labelled Reagent Inventory for Facility. Supervise tittering by users, aliquot reagents for users.
Collaborate with scientists and statisticians in NCI, NIH, and extramural research institutions as appropriate, to advance the research projects and elucidation of cancer-related data.
Collaborate with NIH/NCI expert staff to clarify needs and project objectives through the operation of flow cytometers.
Attend seminars within the National Institute of Health/Center for Cancer Research/Experimental Immunology Branch; pursue additional training, where necessary; participate in discussions at meetings.
Prepare data sets, Tables and Figures for collaborative papers prepared for publication in peer-reviewed journals; assemble and write papers for first-author publication in peer-reviewed papers.
Participate in special committees, panels, and colloquia

DESIRED BACKGROUND

Bachelor of Science degree in Biology, Chemistry or a related discipline.
Minimum of three (3) years of experience in flow cytometry with electronic cell sorting or in a biomedical research laboratory or in operation/troubleshooting of complex biomedical instrumentation.
Strong communications skills, both oral and written.
Excellent computer skills and experience.
Customer Service experience.
Excellent interpersonal, analytical, organizational and time management skills.

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Equal Employment Opportunity (EEO) Information

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Flow Cytometry Specialist